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hydroxyapatite bead ( average diameter)  (Berkeley Advanced Biomaterials)

 
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    Berkeley Advanced Biomaterials hydroxyapatite bead ( average diameter)
    MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to <t>hydroxyapatite</t> (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.
    Hydroxyapatite Bead ( Average Diameter), supplied by Berkeley Advanced Biomaterials, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hydroxyapatite+bead+%28+average+diameter%29/pmc03712787-76-6-13?v=Berkeley+Advanced+Biomaterials
    Average 90 stars, based on 1 article reviews
    hydroxyapatite bead ( average diameter) - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Polyphosphates inhibit extracellular matrix mineralization in MC3T3-E1 osteoblast cultures"

    Article Title: Polyphosphates inhibit extracellular matrix mineralization in MC3T3-E1 osteoblast cultures

    Journal: Bone

    doi: 10.1016/j.bone.2013.01.020

    MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to hydroxyapatite (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.
    Figure Legend Snippet: MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to hydroxyapatite (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.

    Techniques Used: Cell Culture, Staining, Binding Assay, Concentration Assay, Adsorption



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    hydroxyapatite bead ( average diameter)  (Berkeley Advanced Biomaterials)
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    Berkeley Advanced Biomaterials hydroxyapatite bead ( average diameter)
    MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to <t>hydroxyapatite</t> (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.
    Hydroxyapatite Bead ( Average Diameter), supplied by Berkeley Advanced Biomaterials, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hydroxyapatite+bead+%28+average+diameter%29/pmc03712787-76-6-13?v=Berkeley+Advanced+Biomaterials
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    hydroxyapatite bead ( average diameter) - by Bioz Stars, 2026-07
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    hydroxyapatite bead (5 μm average diameter)  (Berkeley Advanced Biomaterials)
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    MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to <t>hydroxyapatite</t> (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.
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    hydroxyapatite (ha) beads (type ii, the average diameter=40 μm)  (Bio-Rad)
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    MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to <t>hydroxyapatite</t> (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.
    Hydroxyapatite (Ha) Beads (Type Ii, The Average Diameter=40 μm), supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hydroxyapatite+bead+%28+average+diameter%29/pm21982903-45-0-12?v=Bio-Rad
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    Image Search Results


    MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to hydroxyapatite (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.

    Journal: Bone

    Article Title: Polyphosphates inhibit extracellular matrix mineralization in MC3T3-E1 osteoblast cultures

    doi: 10.1016/j.bone.2013.01.020

    Figure Lengend Snippet: MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to hydroxyapatite (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.

    Article Snippet: Hydroxyapatite binding assay A 20 mg/ml stock hydroxyapatite bead (5 μm average diameter, Berkeley Advanced Biomaterials, Berkeley, CA, USA) slurry was generated by washing the beads as received from the supplier three times in Tris-buffered saline, pH 7.0 (TBS), and then reconstituting in TBS.

    Techniques: Cell Culture, Staining, Binding Assay, Concentration Assay, Adsorption